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   Of Interest

   NEW! 
 
      MCSG Screens 

“Crystal Former is very easy to set up and handle during follow-up visual inspection. It offers unique capabilities of gradient screening.  It speeds up the whole process of crystallization. For some projects it helps us identify novel crystallization hits.”

Dr Nickolay Chirgadze
University Health Network, Toronto

   Upcoming Events:
 
ICCBM 13
September 12-16th, 2010
Dublin, Ireland
 
PSDI Meeting
November 14-17th, 2010
Oxford, UK


 

Microlytic's page on Facebook

Terese Bergfors: 
“We use the Crystal
Former as part of our
overall crystallization
strategy..."
 


 

Whitepapers

From screen to Structure using the Crystal Former
Researchers from Brookhaven National Labs demonstrate how to go directly from initial crystallization screening to high resolution structure for a protein using the Crystal Former. 
 
  

 Improved crystallization hit rate. 

Researchers from Brookhaven National Labs demonstrate improved crystallization hit rates using the Crystal Former for initial sparse screening compared to sitting drop vapor diffusion. 
 
 
UV fluorescence
Researchers from Rockefeller University and Jan Scientific show how crystals can be imaged in Crystal Former channels as well as detected using the UV fluorescence of tryptophan. 
 

Detection with the PX Scanner
In collaboration with Oxford Diffraction, crystals are detected and analysed in the Crystal Former via PX Scanner.
 
 
 
Crystallization of protein targets from the Midwest Center for Structural Genomics.
A set of proteins that had failed the vapor diffusion based pipeline of the MCSG were selected for crystallization using the Crysta Formers and the SmartScreen.
 
 
Visualization of macromolecular crystals using the RockImager from Formulatrix
 The Crystal Formers comprise a crystallization system that is highly flexible with regard to experimental format while retaining the necessary features for full compatibility with currently available robotics, such as the RockImager. 
 
 
 
 
Product sheets
 
Crystal Former 
Get an overview of the Crystal Former and the advantages it offers to protein crystallographers. 
 
 
SmartScreen
A new sparse matrix screen designed to give researchers the most crystal hits using only 48 conditions in the Crystal Former. 
 
 
 
PurePEGs
A new PEG based screen that utilized ultra pure PEGs and features many different MW PEGs in each condition resulting in diffusive waves of different PEGs through the Crystal Former channels. 
 
PurePEGs Product Sheet
 
 
MCSG Suites NEW!! 
New MCSG suites offered in 1.7 ml deep well block format offered by Microlytic.
 
 
Salt in-Salt out
A screen designed to take advantage of the benefits of the XZ Dialysis system. The Salt in-Salt out screen samples 50% in the Salting in region of the phase diagram and 50% in the Salting out region.

 
 
 
Formulation Sheets
 
SmartScreen
Download Formulation Sheets in PDF or Excel format.
 
 

PurePEGS
Download Formulation Sheet in PDF format
 
 
Salt in- Salt out
Download Formulation Sheet in Excel Format
 

MSDS Sheets


PurePEGs Screen components:
PEG Smear
PEG 300

 

Automation
 
Mosquito script for loading the Crystal Former 
Step by step guide for automated setup of the Crystal Former using the Mosquito system. 
NOTE: Mosquito .plate file for the Crystal Former is emailed per costumer request - please contact Microlytic to get script file. 
 
  
  
Suggested Protocols
 
How to harvest crystals from the Crystal Former.
 
User manuals
 
How to evaluate the Crystal Former
2 page description of how to conduct a well designed evaluation of how the Crystal Former compares to your current workflow.

 
 
Crystal Former user manual
Step by step manual for setting up the Crystal Former
 
 
 
Harvesting from the Crystal Former 
Step by step presentation on how to harvest crystals formed within the Crystal Former channels.
 


XZ dialysis plate user manual
Step by step manual for setting up the XZ dialysis plate.


Videos
 

 

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Phone: +1 781 376 0780  |  Fax: +1 781 376 0785  |  © 2009 Microlytic ApS